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Based on the residual monophenolase activity towards phenol and tyrosol in Asn240Lys/Leu244Arg and Asn240Thr/Leu244Arg, we propose that PPOs may show activity towards non-standard monophenolic substrates (secondary metabolites of Juglans regia ) despite not accepting tyrosine or tyramine

For in vitro cytotoxicity assays where benzyl alcohol vehicle effects could confound results, include matched Bacteriostatic Water vehicle controls at the same final benzyl alcohol concentration as the peptide treatment to distinguish compound-specific from vehicle-associated biological effects

doi: 10.1097/CJI.0b013e3182280db1

Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects

This lifts the fat layer away from the muscle underneath